Bone marrow mesenchymal stem cells seeded into an allogeneic scaffold repair critical-sized iliac defects in sheep

doi:10.3969/j.issn.2095-4344.2013.16.002

Abstract

Abstract:

BACKGROUND: Nowadays, the research regarding tissue engineering bone materials and construction methods continues to heat up, and how to repair large bone defects is still difficult in the clinic.
OBJECTIVE: To compare the effects of tissue engineering bones with allogeneic scaffold material, β-tricalcium phosphate material and basic fibroblast growth factor lentiviral vector-transfected bone marrow mesenchymal stem cells on the repairing of critical-sized iliac defects in sheep.
METHODS: The sheep bone marrow mesenchymal stem cells were in vitro induced and cultured to the third generation, and then the basic fibroblast growth factor lentiviral vector was constructed. The allogeneic scaffold materials with transfected and untransfected bone marrow mesenchymal stem cells, simple allogeneic scaffold materials and β-tricalcium phosphate materials were implanted into the sheep model of critical-sized iliac defects (15 mm×10 mm×10 mm). Imaging, histology, and scanning electron microscopy observations were performed at 4, 8 and 12 weeks after repairing.
RESULTS AND CONCLUSION: The repairing effect of allogeneic scaffold materials with basic fibroblast growth factor lentiviral vector transfected bone marrow mesenchymal stem cells on sheep critical-sized iliac defects was better than that of untransfected allogeneic scaffold materials, simple allogeneic scaffold materials and β-tricalcium phosphate materials; the effect of untransfected allogeneic scaffold materials was better than that of simple allogeneic scaffold materials and β-tricalcium phosphate materials; the degradation rate of simple allogeneic scaffold materials was higher than that of β-tricalcium phosphate materials. The tissue engineering bone constructed using allogeneic scaffold materials with basic fibroblast growth factor lentiviral vector-transfected bone marrow mesenchymal stem cells can meet the requirement of bone repairing, and it can guide the new bone formation with good biocompatibility and cannot interfere with bone tissue reconstruction and modeling.

Key words: biomaterials, tissue-engineered bone materials, critical-sized defect, basic fibroblast growth factor, lentiviral vector, bone marrow mesenchymal stem cells, tissue engineering bone, bone scaffold materials, β-tricalcium phosphate materials, National Natural Science Foundation of China

CLC Number:

R318

Yang Nan, He Hui-yu, Hu Yang, Yang Chuan-bo. Bone marrow mesenchymal stem cells seeded into an allogeneic scaffold repair critical-sized iliac defects in sheep[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(16): 2859-2868.

Figures/Tables 8

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